More than 2 billion people are infected with HBV worldwide, with an estimated 320 thousand deaths occurring every year, of which approximately 30-50% are due to HCC. Currently, hepatitis B virus (HBV) is commonly recognized as one of the main causes of HCC. Primary hepatocellular carcinoma (HCC) is highly malignant. HBV may be involved in the occurrence and development of HCC by upregulating CtBP2 expression. This activation effect was enhanced by the increase in the dose of the X gene, showing metrological dependence. The HBV X gene significantly activated CtBP2 gene promoter activity, and CtBP2 mRNA and protein expression were upregulated by the HBV X gene. pHBV1.3 upregulated CtBP2 mRNA and protein expression. CtBP2 expression was higher in HepG2.2.15 cells integrated with the HBV genome than in HepG2 cells. CtBP2 expression was higher in HBV-related HCC tissues than in paracancerous tissues. The HBV infectious clone pHBV1.3 and plasmids expressing a single gene of the HBV genome were cotransfected with the CtBP2 gene promoter pG元-CtBP2 into the human hepatoma cell line HepG2, and luciferase activity was determined using a luminometer.
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Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting were used to evaluate the CtBP2 mRNA and protein expression levels in tissues and cells. The aim of this study was to investigate the effect of HBV on CtBP2 expression and to explore its mechanism. Carboxyl-terminal-binding protein 2 (CtBP2) plays an important role in tumorigenesis and progression. Hepatitis B virus (HBV) infection causes acute and chronic liver diseases that can eventually develop into cirrhosis and hepatocellular carcinoma (HCC), but the carcinogenesis of HBV is not fully understood.
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restitura and potential novel targets for pest control strategies.Background. Our findings provided a foundation for future studies on the molecular mechanisms controlling the olfactory system in C. Our results suggested that these proteins might play a key role in foraging, seeking mates, and host recognition in C. CresOBP10 showed a remarkably high expression in legs compared to other studied insects. The expression level of CresGOBP2 in female antennae was approximately two times higher than in males, and two pheromone binding proteins PBPs (CresPBP1 and -PBP3) and three OBPs (CresOBP9, −10, and −16) were more highly enriched in male antennae than in female antennae.
![pbp3 protein rt qprc pbp3 protein rt qprc](https://ars.els-cdn.com/content/image/1-s2.0-S1744117X18300765-ga1.jpg)
restitura sexes by using reverse transcription PCR and quantitative real time PCR (RT-qPCR). Furthermore, we systematically analyzed expression patterns of eight OBPs from different tissues of both C. A total of 165 transcripts were identified, including 43 transcripts encoding putative odorant-binding proteins (OBPs), 13 chemosensory proteins (CSPs), 78 odorant receptors (ORs), 15 ionotropic receptors (IRs), 13 gustatory receptors (GRs), and 3 sensory neuron membrane proteins (SNMPs).
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We constructed an antennal transcriptome using Illumina Hiseq 2500™ sequencing and characterized the expression profiles of odorant binding proteins for better understanding of the olfactory receptive system and the role of putative olfactory proteins in C. Antennal transcriptome analysis and expression profiles of odorant binding proteins in Clostera restitura Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.674), Pub Date : , DOI: 10.1016/j.cbd.2018.12.002 Tianzi Gu, Kairu Huang, Shuo Tian, Yuhang Sun, Hui Li, Cong Chen, Dejun HaoĬlostera restitura Walker (Lepidoptera: Notodontidae) is one of the most destructive defoliators of poplars in China.